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mouse anti pi3k  (Proteintech)


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    Proteintech mouse anti pi3k
    Mouse Anti Pi3k, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 256 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti pi3k/product/Proteintech
    Average 96 stars, based on 256 article reviews
    mouse anti pi3k - by Bioz Stars, 2026-04
    96/100 stars

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    GaELNs Treat Periodontitis by Activating the <t>PHGDH/PI3K/AKT</t> Pathway.( A ). Volcano plot showing differentially expressed genes between the LPS + GaELNs and LPS groups. ( B ). Venn diagram illustrating the overlap among gene sets “GaELNs vs Control upregulated” “LPS vs Control downregulated” and “LPS + GaELNs vs Control upregulated” with 12 genes common to all three groups. ( C ). KEGG pathway analysis of genes upregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation. Down: downregulated. ( D ). KEGG pathway analysis of genes downregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation and metabolism. Up: upregulated. ( E ). CCK-8 showing cell viability changes over time with different concentrations of NCT-503 co-cultured with HGF. ( F ). qPCR analysis of PHGDH mRNA levels in HGF under different treatment conditions. ( G ). WB analysis of PHGDH, PI3K, p-PI3K, AKT, p-AKT and GAPDH expression levels in whole cell lysates. ( H – J ). Relative protein expression levels of PHGDH/GAPDH, p-PI3K/PI3K and p-AKT/AKT based on band intensities (n=3). ( K ). Immunofluorescence staining for PHGDH and PI3K in tissue sections of the first molar of the mouse mandible. Gray solid lines were used for fluorescence colocalization analysis of PHGDH and PI3K. Scale bar = 200 μm. ( L and M ). Semi-quantitative analysis of red fluorescence for PHGDH and green fluorescence for PI3K (n=5). ( N ). Colocalization analysis of PHGDH red fluorescence and PI3K green fluorescence along the grayscale line. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
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    GaELNs Treat Periodontitis by Activating the PHGDH/PI3K/AKT Pathway.( A ). Volcano plot showing differentially expressed genes between the LPS + GaELNs and LPS groups. ( B ). Venn diagram illustrating the overlap among gene sets “GaELNs vs Control upregulated” “LPS vs Control downregulated” and “LPS + GaELNs vs Control upregulated” with 12 genes common to all three groups. ( C ). KEGG pathway analysis of genes upregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation. Down: downregulated. ( D ). KEGG pathway analysis of genes downregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation and metabolism. Up: upregulated. ( E ). CCK-8 showing cell viability changes over time with different concentrations of NCT-503 co-cultured with HGF. ( F ). qPCR analysis of PHGDH mRNA levels in HGF under different treatment conditions. ( G ). WB analysis of PHGDH, PI3K, p-PI3K, AKT, p-AKT and GAPDH expression levels in whole cell lysates. ( H – J ). Relative protein expression levels of PHGDH/GAPDH, p-PI3K/PI3K and p-AKT/AKT based on band intensities (n=3). ( K ). Immunofluorescence staining for PHGDH and PI3K in tissue sections of the first molar of the mouse mandible. Gray solid lines were used for fluorescence colocalization analysis of PHGDH and PI3K. Scale bar = 200 μm. ( L and M ). Semi-quantitative analysis of red fluorescence for PHGDH and green fluorescence for PI3K (n=5). ( N ). Colocalization analysis of PHGDH red fluorescence and PI3K green fluorescence along the grayscale line. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Garlic-Derived Exosome-Like Nanovesicles: A Promising Natural Nanotherapy for Periodontitis via PHGDH/PI3K/AKT-Mediated Metabolic and Inflammatory Regulation

    doi: 10.2147/IJN.S510417

    Figure Lengend Snippet: GaELNs Treat Periodontitis by Activating the PHGDH/PI3K/AKT Pathway.( A ). Volcano plot showing differentially expressed genes between the LPS + GaELNs and LPS groups. ( B ). Venn diagram illustrating the overlap among gene sets “GaELNs vs Control upregulated” “LPS vs Control downregulated” and “LPS + GaELNs vs Control upregulated” with 12 genes common to all three groups. ( C ). KEGG pathway analysis of genes upregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation. Down: downregulated. ( D ). KEGG pathway analysis of genes downregulated in the LPS + GaELNs group compared to the Control group. The red boxes highlight signaling pathways associated with inflammation and metabolism. Up: upregulated. ( E ). CCK-8 showing cell viability changes over time with different concentrations of NCT-503 co-cultured with HGF. ( F ). qPCR analysis of PHGDH mRNA levels in HGF under different treatment conditions. ( G ). WB analysis of PHGDH, PI3K, p-PI3K, AKT, p-AKT and GAPDH expression levels in whole cell lysates. ( H – J ). Relative protein expression levels of PHGDH/GAPDH, p-PI3K/PI3K and p-AKT/AKT based on band intensities (n=3). ( K ). Immunofluorescence staining for PHGDH and PI3K in tissue sections of the first molar of the mouse mandible. Gray solid lines were used for fluorescence colocalization analysis of PHGDH and PI3K. Scale bar = 200 μm. ( L and M ). Semi-quantitative analysis of red fluorescence for PHGDH and green fluorescence for PI3K (n=5). ( N ). Colocalization analysis of PHGDH red fluorescence and PI3K green fluorescence along the grayscale line. All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: The following antibodies were used for WB or immunofluorescence labeling: HO-1 Rabbit mAb (A21911), Nrf2 Rabbit mAb (A21176), NQO1 Rabbit mAb (A23486), GAPDH Rabbit mAb (A19056), PHGDH Rabbit mAb (A22129), mTOR Rabbit pAb (A2445), NF-kB p65 Rabbit mAb (A19653), TOM20 Rabbit mAb (A19403), TFAM Rabbit mAb (A3173), FITC-conjugated Goat anti-Rabbit IgG (AS011), Cy3-conjugated Goat anti-Mouse IgG (AS008) were purchased from ABclonal Biotech Co., Ltd (Wuhan, China); anti-VEGFA Antibody (ab1316) and anti-p-NF-kB p65 Antibody (ab32536) were obtained from Abcam (Cambridge, UK); PI3K p85 Mouse mAb (#13666) p-PI3K p85 (#4228), Akt Rabbit mAb (#4691), p-Akt Rabbit mAb (#4060) and p-mTOR Antibody (#2971) were sourced from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Control, Protein-Protein interactions, CCK-8 Assay, Cell Culture, Expressing, Immunofluorescence, Staining, Fluorescence

    GaELNs Promote Cell Proliferation and Migration by Upregulating mTOR and VEGF Expression via the PHGDH/PI3K/AKT Pathway. ( A ). WB analysis of mTOR, p-mTOR, VEGF and GAPDH expression levels in whole cell lysates. ( B and C ). Relative protein expression levels of p-mTOR/mTOR and VEGF/GAPDH based on band intensities (n=3). ( D ). CCK-8 assay showing changes in HGF cell viability following the addition of GaELNs and NCT-503. ( E ). Flow cytometry analysis of GaELNs and NCT-503 effects on the proliferation of CFDA-SE-labeled HGF. ( F ). Scratch assay evaluating the impact of GaELNs and NCT-503 on HGF migration. Scale bar = 50 μm. ( G ). Immunofluorescence analysis of VEGF expression in HGF following treatment with GaELNs and NCT-503. Scale bar = 20 μm. ( H ). Semi-quantitative analysis of cell migration distance (n=3). ( I ). Semi-quantitative analysis of VEGF fluorescence intensity (n=3). All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Garlic-Derived Exosome-Like Nanovesicles: A Promising Natural Nanotherapy for Periodontitis via PHGDH/PI3K/AKT-Mediated Metabolic and Inflammatory Regulation

    doi: 10.2147/IJN.S510417

    Figure Lengend Snippet: GaELNs Promote Cell Proliferation and Migration by Upregulating mTOR and VEGF Expression via the PHGDH/PI3K/AKT Pathway. ( A ). WB analysis of mTOR, p-mTOR, VEGF and GAPDH expression levels in whole cell lysates. ( B and C ). Relative protein expression levels of p-mTOR/mTOR and VEGF/GAPDH based on band intensities (n=3). ( D ). CCK-8 assay showing changes in HGF cell viability following the addition of GaELNs and NCT-503. ( E ). Flow cytometry analysis of GaELNs and NCT-503 effects on the proliferation of CFDA-SE-labeled HGF. ( F ). Scratch assay evaluating the impact of GaELNs and NCT-503 on HGF migration. Scale bar = 50 μm. ( G ). Immunofluorescence analysis of VEGF expression in HGF following treatment with GaELNs and NCT-503. Scale bar = 20 μm. ( H ). Semi-quantitative analysis of cell migration distance (n=3). ( I ). Semi-quantitative analysis of VEGF fluorescence intensity (n=3). All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: The following antibodies were used for WB or immunofluorescence labeling: HO-1 Rabbit mAb (A21911), Nrf2 Rabbit mAb (A21176), NQO1 Rabbit mAb (A23486), GAPDH Rabbit mAb (A19056), PHGDH Rabbit mAb (A22129), mTOR Rabbit pAb (A2445), NF-kB p65 Rabbit mAb (A19653), TOM20 Rabbit mAb (A19403), TFAM Rabbit mAb (A3173), FITC-conjugated Goat anti-Rabbit IgG (AS011), Cy3-conjugated Goat anti-Mouse IgG (AS008) were purchased from ABclonal Biotech Co., Ltd (Wuhan, China); anti-VEGFA Antibody (ab1316) and anti-p-NF-kB p65 Antibody (ab32536) were obtained from Abcam (Cambridge, UK); PI3K p85 Mouse mAb (#13666) p-PI3K p85 (#4228), Akt Rabbit mAb (#4691), p-Akt Rabbit mAb (#4060) and p-mTOR Antibody (#2971) were sourced from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Migration, Expressing, CCK-8 Assay, Flow Cytometry, Labeling, Wound Healing Assay, Immunofluorescence, Fluorescence

    GaELNs Reduce Inflammation and Oxidative Stress and Improve Mitochondrial Function by Downregulating p65 and Upregulating Nrf2 via the PHGDH/PI3K/AKT Pathway. ( A ). WB analysis of p65, p-p65, Nrf2 and GAPDH expression levels in whole cell lysates. ( B and C ). Relative protein expression levels of p-p65/p65 and Nrf2/GAPDH based on band intensities (n=3). ( D ). DCFH-DA staining to assess intracellular ROS levels in HGF under different treatments. Scale bar = 20 μm. ( E ). Immunofluorescence and Mito-Tractor staining to observe PHGDH expression and mitochondrial morphology in HGF following different treatments. Scale bar = 10 μm. ( F ). MitoSOX fluorescence staining to measure mitochondrial ROS levels in HGF under different treatments. Scale bar = 10 μm ( G ). TMRM fluorescence staining to assess mitochondrial membrane potential in HGF under different treatments. Scale bar = 10 μm ( H ). Flow cytometry analysis of MitoSOX fluorescence intensity. ( I ). Flow cytometry analysis of TMRM fluorescence intensity. ( J ). Ratio of cytoplasmic ND1 to 18s rRNA levels (n=3). ( K ). WB analysis of TOM20, TFAM and GAPDH expression levels in whole cell lysates. ( L and M ). Relative protein expression levels of TOM20/GAPDH and TFAM/GAPDH based on band intensities (n=3). All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Journal: International Journal of Nanomedicine

    Article Title: Garlic-Derived Exosome-Like Nanovesicles: A Promising Natural Nanotherapy for Periodontitis via PHGDH/PI3K/AKT-Mediated Metabolic and Inflammatory Regulation

    doi: 10.2147/IJN.S510417

    Figure Lengend Snippet: GaELNs Reduce Inflammation and Oxidative Stress and Improve Mitochondrial Function by Downregulating p65 and Upregulating Nrf2 via the PHGDH/PI3K/AKT Pathway. ( A ). WB analysis of p65, p-p65, Nrf2 and GAPDH expression levels in whole cell lysates. ( B and C ). Relative protein expression levels of p-p65/p65 and Nrf2/GAPDH based on band intensities (n=3). ( D ). DCFH-DA staining to assess intracellular ROS levels in HGF under different treatments. Scale bar = 20 μm. ( E ). Immunofluorescence and Mito-Tractor staining to observe PHGDH expression and mitochondrial morphology in HGF following different treatments. Scale bar = 10 μm. ( F ). MitoSOX fluorescence staining to measure mitochondrial ROS levels in HGF under different treatments. Scale bar = 10 μm ( G ). TMRM fluorescence staining to assess mitochondrial membrane potential in HGF under different treatments. Scale bar = 10 μm ( H ). Flow cytometry analysis of MitoSOX fluorescence intensity. ( I ). Flow cytometry analysis of TMRM fluorescence intensity. ( J ). Ratio of cytoplasmic ND1 to 18s rRNA levels (n=3). ( K ). WB analysis of TOM20, TFAM and GAPDH expression levels in whole cell lysates. ( L and M ). Relative protein expression levels of TOM20/GAPDH and TFAM/GAPDH based on band intensities (n=3). All data are expressed as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

    Article Snippet: The following antibodies were used for WB or immunofluorescence labeling: HO-1 Rabbit mAb (A21911), Nrf2 Rabbit mAb (A21176), NQO1 Rabbit mAb (A23486), GAPDH Rabbit mAb (A19056), PHGDH Rabbit mAb (A22129), mTOR Rabbit pAb (A2445), NF-kB p65 Rabbit mAb (A19653), TOM20 Rabbit mAb (A19403), TFAM Rabbit mAb (A3173), FITC-conjugated Goat anti-Rabbit IgG (AS011), Cy3-conjugated Goat anti-Mouse IgG (AS008) were purchased from ABclonal Biotech Co., Ltd (Wuhan, China); anti-VEGFA Antibody (ab1316) and anti-p-NF-kB p65 Antibody (ab32536) were obtained from Abcam (Cambridge, UK); PI3K p85 Mouse mAb (#13666) p-PI3K p85 (#4228), Akt Rabbit mAb (#4691), p-Akt Rabbit mAb (#4060) and p-mTOR Antibody (#2971) were sourced from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Expressing, Staining, Immunofluorescence, Fluorescence, Membrane, Flow Cytometry